TEV 蛋白酶（切His tag）10 Units/μl TEV Protease 详情
| 货 号 | SB-EGR218S | ||
|---|---|---|---|
| 规 格 | 0.1ml | ||
| 品 牌 | 圣尔生物share-bio | ||
| 产品编号 | 名称 | 包装 | 品牌 | 目录价 | 促销价 | 货期 | 数量 | 购物车 |
| SB-EGR218S | TEV 蛋白酶（切His tag）10 Units/μl TEV Protease | 0.1ml | 圣尔生物share-bio | ¥175.00 |
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| SB-EGR218S | TEV 蛋白酶（切His tag）10 Units/μl TEV Protease | 1mL | 圣尔生物share-bio | ¥1200.00 |
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TEV 蛋白酶（切His tag）10 Units/μl TEV Protease 介绍
| 产品详情 | <p class="MsoNormal" style="text-indent: 21.0000pt; line-height: 20.0000pt; mso-line-height-rule: exactly;"><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: 仿宋; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;">TEV <span style="font-family: 仿宋;">蛋白酶是一种高度特异性的半胱氨酸蛋白酶。催化效率最高的 </span><span style="font-family: 'Times New Roman';">TEV </span><span style="font-family: 仿宋;">蛋白酶识别序列是 </span><span style="font-family: 'Times New Roman';">ENLYFQ▼S</span><span style="font-family: 仿宋;">；不过，</span><span style="font-family: 'Times New Roman';">P1'</span><span style="font-family: 仿宋;">位置的氨基酸也可以是 </span><span style="font-family: 'Times New Roman';">G</span><span style="font-family: 仿宋;">、</span><span style="font-family: 'Times New Roman';">A</span><span style="font-family: 仿宋;">、</span><span style="font-family: 'Times New Roman';">M</span><span style="font-family: 仿宋;">、</span><span style="font-family: 'Times New Roman';">C </span><span style="font-family: 仿宋;">或 </span><span style="font-family: 'Times New Roman';">H</span><span style="font-family: 仿宋;">。它通常用于去除亲和纯化标签，如融合蛋白中的麦芽糖结合蛋白（</span><span style="font-family: 'Times New Roman';">MBP</span><span style="font-family: 仿宋;">）、多聚组氨酸标签（</span><span style="font-family: 'Times New Roman';">His-Tag</span><span style="font-family: 仿宋;">）或者其他亲和纯化标签。</span><span style="font-family: 'Times New Roman';">TEV </span><span style="font-family: 仿宋;">蛋白酶自身带有一个 </span><span style="font-family: 'Times New Roman';">6xHis </span><span style="font-family: 仿宋;">标记，便于从使用镍亲和树脂的反应中去除，而且本产品经过设计改造可提高热稳定性并减少自溶。建议把</span><span style="font-family: 'Times New Roman';">MBP</span></span><span style="mso-spacerun: 'yes'; font-family: 仿宋; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;">、</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: 仿宋; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;">His</span><span style="mso-spacerun: 'yes'; font-family: 仿宋; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;"><span style="font-family: 仿宋;">或</span> <span style="font-family: 'Times New Roman';">GST</span></span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: 仿宋; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;"><span style="font-family: 仿宋;">等标签设计在融合蛋白的</span>N<span style="font-family: 仿宋;">端，并在亲和标签与目的蛋白之间设计加入</span><span style="font-family: 'Times New Roman';">TEV </span></span><span style="mso-spacerun: 'yes'; font-family: 仿宋; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;">蛋白酶</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: 仿宋; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;"><span style="font-family: 仿宋;">专一性识别与酶切的上述序列。</span></span></p> <p class="MsoNormal" style="margin-top: 7.8000pt; margin-bottom: 7.8000pt; mso-para-margin-top: 0.5000gd; mso-para-margin-bottom: 0.5000gd; line-height: 20.0000pt; mso-line-height-rule: exactly;"><strong><span style="font-family: 'Times New Roman'; font-size: 12pt;"><span style="font-family: 仿宋;">推荐反应条件</span></span></strong></p> <p class="MsoNormal" style="line-height: 20.0000pt; mso-line-height-rule: exactly;"><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: 仿宋; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;">1X TEV Protease Reaction Buffer, 30℃ <span style="font-family: 仿宋;">孵育 </span><span style="font-family: 'Times New Roman';">1 </span><span style="font-family: 仿宋;">小时，或 </span><span style="font-family: 'Times New Roman';">4℃ </span><span style="font-family: 仿宋;">过夜孵育。</span></span></p> <p class="MsoNormal" style="text-indent: 21.0000pt; line-height: 20.0000pt; mso-line-height-rule: exactly;"><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: 仿宋; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;"><span style="font-family: 仿宋;">在实际操作过程中，为尽量保留目的蛋白的结构和生物活性，建议在</span>4℃<span style="font-family: 仿宋;">用</span><span style="font-family: 'Times New Roman';">TEV Protease</span><span style="font-family: 仿宋;">酶切过夜</span></span><span style="mso-spacerun: 'yes'; font-family: 仿宋; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;">。</span></p> <p class="MsoNormal" style="margin-top: 7.8000pt; margin-bottom: 7.8000pt; mso-para-margin-top: 0.5000gd; mso-para-margin-bottom: 0.5000gd; line-height: 20.0000pt; mso-line-height-rule: exactly;"><strong><span style="font-family: 仿宋; font-size: 12pt;">优势和特性</span></strong></p> <p class="15" style="margin-left: 18.0000pt; text-indent: -18.0000pt; mso-char-indent-count: 0.0000; line-height: 20.0000pt; mso-line-height-rule: exactly; mso-list: l0 level1 lfo1;"><!--[if !supportLists]--><span style="font-family: 'Times New Roman'; mso-fareast-font-family: 仿宋; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;">1. </span><!--[endif]--><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: 仿宋; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;">TEV </span><span style="mso-spacerun: 'yes'; font-family: 仿宋; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;">蛋白酶分</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: 仿宋; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;"><span style="font-family: 仿宋;">子量大小约</span>28kDa<span style="font-family: 仿宋;">，纯度：</span><span style="font-family: 'Times New Roman';">≥95%</span><span style="font-family: 仿宋;">。</span><span style="font-family: 'Times New Roman';">10X </span></span><span style="mso-spacerun: 'yes'; font-family: 仿宋; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;"><span style="font-family: 'Times New Roman';">P</span></span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: 仿宋; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;">rotease Reaction Buffer<span style="font-family: 仿宋;">组成为：</span><span style="font-family: 'Times New Roman';">500mM Tris-HCl, 500mM NaCl, 5mM EDTA, 10mM DTT, pH 8.0</span><span style="font-family: 仿宋;">。</span></span></p> <p class="15" style="margin-left: 18.0000pt; text-indent: -18.0000pt; mso-char-indent-count: 0.0000; line-height: 20.0000pt; mso-line-height-rule: exactly; mso-list: l0 level1 lfo1;"><!--[if !supportLists]--><span style="font-family: 'Times New Roman'; mso-fareast-font-family: 仿宋; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;">2. </span><!--[endif]--><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: 仿宋; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;">TEV </span><span style="mso-spacerun: 'yes'; font-family: 仿宋; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;">蛋白酶</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: 仿宋; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;"><span style="font-family: 仿宋;">突出的优点是在</span>400mM<span style="font-family: 仿宋;">咪唑中仍有较高活力，</span></span><span style="mso-spacerun: 'yes'; font-family: 仿宋; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;">因此</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: 仿宋; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;"><span style="font-family: 仿宋;">可直接将</span>TEV </span><span style="mso-spacerun: 'yes'; font-family: 仿宋; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;">蛋白酶</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: 仿宋; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;"><span style="font-family: 仿宋;">加入含高浓度咪唑的刚刚纯化的目的蛋白溶液中，在</span>4℃<span style="font-family: 仿宋;">边透析去除咪唑边进行酶切。</span></span></p> <p class="15" style="margin-left: 18.0000pt; text-indent: -18.0000pt; mso-char-indent-count: 0.0000; line-height: 20.0000pt; mso-line-height-rule: exactly; mso-list: l0 level1 lfo1;"><!--[if !supportLists]--><span style="font-family: 'Times New Roman'; mso-fareast-font-family: 仿宋; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;">3. </span><!--[endif]--><span style="mso-spacerun: 'yes'; font-family: 仿宋; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;">本产品</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: 仿宋; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;"><span style="font-family: 仿宋;">比较适用于酶切带</span>His<span style="font-family: 仿宋;">标签的蛋白，因为酶切后没有完全酶切的</span><span style="font-family: 'Times New Roman';">His</span><span style="font-family: 仿宋;">标签重组蛋白、切除下来的</span><span style="font-family: 'Times New Roman';">His</span><span style="font-family: 仿宋;">标签以及</span><span style="font-family: 'Times New Roman';">TEV </span></span><span style="mso-spacerun: 'yes'; font-family: 仿宋; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;">蛋白酶</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: 仿宋; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;"> (His-tag)<span style="font-family: 仿宋;">都可以结合于镍柱而被除去，穿柱液中则含有所需的靶蛋白。</span></span></p> <p class="15" style="margin-left: 18.0000pt; text-indent: -18.0000pt; mso-char-indent-count: 0.0000; line-height: 20.0000pt; mso-line-height-rule: exactly; mso-list: l0 level1 lfo1;"><!--[if !supportLists]--><span style="font-family: 'Times New Roman'; mso-fareast-font-family: 仿宋; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;">4. </span><!--[endif]--><span style="mso-spacerun: 'yes'; font-family: 仿宋; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;"><span style="font-family: 'Times New Roman';">TEV </span><span style="font-family: 仿宋;">蛋白酶的</span></span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: 仿宋; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;"><span style="font-family: 仿宋;">活性不会被常见的丝氨酸蛋白酶抑制剂</span></span><span style="mso-spacerun: 'yes'; font-family: 仿宋; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;">所抑制，</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: 仿宋; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;"><span style="font-family: 仿宋;">如</span>PMSF<span style="font-family: 仿宋;">、</span><span style="font-family: 'Times New Roman';">AEBSF</span><span style="font-family: 仿宋;">、</span><span style="font-family: 'Times New Roman';">bestatin</span><span style="font-family: 仿宋;">、</span><span style="font-family: 'Times New Roman';">pepstatin</span><span style="font-family: 仿宋;">和</span><span style="font-family: 'Times New Roman';">EDTA</span></span><span style="mso-spacerun: 'yes'; font-family: 仿宋; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;">等。但</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: 仿宋; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;"><span style="font-family: 仿宋;">如果融合蛋白样品中含有</span> > 2 M <span style="font-family: 仿宋;">尿素、</span><span style="font-family: 'Times New Roman';">> 0.5 M </span><span style="font-family: 仿宋;">盐酸胍、</span><span style="font-family: 'Times New Roman';">pH < 6 </span><span style="font-family: 仿宋;">或</span></span><span style="mso-spacerun: 'yes'; font-family: 仿宋; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;"> <span style="font-family: 'Times New Roman';">></span></span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: 仿宋; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;"> 9</span><span style="mso-spacerun: 'yes'; font-family: 仿宋; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;"><span style="font-family: 'Times New Roman';">,</span></span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: 仿宋; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;"> <span style="font-family: 仿宋;">或</span></span><span style="mso-spacerun: 'yes'; font-family: 仿宋; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;">含有</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: 仿宋; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;"><span style="font-family: 仿宋;">半胱氨酸蛋白酶抑制剂，则需要在</span> TEV <span style="font-family: 仿宋;">蛋白酶裂解前透析融合蛋白。</span></span></p> <p class="15" style="margin-left: 18.0000pt; text-indent: -18.0000pt; mso-char-indent-count: 0.0000; line-height: 20.0000pt; mso-line-height-rule: exactly; mso-list: l0 level1 lfo1;"><!--[if !supportLists]--><span style="font-family: 'Times New Roman'; mso-fareast-font-family: 仿宋; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;">5. </span><!--[endif]--><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: 仿宋; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;">TEV <span style="font-family: 仿宋;">蛋白酶可高浓度使用，不会发生非特异性蛋白水解</span></span><span style="mso-spacerun: 'yes'; font-family: 仿宋; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;">，</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: 仿宋; font-size: 12.0000pt; mso-font-kerning: 1.0000pt;"><span style="font-family: 仿宋;">反应可以线性放大，以适应更大的反应体积。</span></span></p> |
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| 储存条件: | -20℃ |
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