快速内切酶KpnI（同裂酶Asp718I） 详情
| 货 号 | SB-EGR444S | ||
|---|---|---|---|
| 规 格 | 200 rxns | ||
| 品 牌 | 圣尔生物share-bio | ||
| 产品编号 | 名称 | 包装 | 品牌 | 目录价 | 促销价 | 货期 | 数量 | 购物车 |
| SB-EGR444S | 快速内切酶KpnI（同裂酶Asp718I） | 200 rxns | 圣尔生物share-bio | ¥192.00 |
|
快速内切酶KpnI（同裂酶Asp718I） 介绍
| 产品详情 | <p class="MsoNormal" style="margin-bottom: 0.0000pt; text-indent: 24.0000pt; mso-char-indent-count: 2.0000; text-autospace: ideograph-numeric; mso-pagination: widow-orphan; text-align: justify; text-justify: inter-ideograph; line-height: 150%;" align="justify"><span style="mso-spacerun: 'yes'; font-family: Arial; mso-fareast-font-family: 微软雅黑; line-height: 150%; color: #191919; font-size: 12.0000pt;"><span style="font-family: 微软雅黑;">圣尔</span></span><span style="mso-spacerun: 'yes'; font-family: Arial; mso-fareast-font-family: 微软雅黑; line-height: 150%; color: #191919; font-size: 12.0000pt;"><span style="font-family: 微软雅黑;">快速内切酶是一系列经过基因工程重组、能够在</span> 5~15 <span style="font-family: 微软雅黑;">分钟内精确完成 </span>DNA <span style="font-family: 微软雅黑;">切割的限制性内切酶，适用于质粒 </span>DNA<span style="font-family: 微软雅黑;">、 </span>PCR <span style="font-family: 微软雅黑;">产物或基因组 </span>DNA <span style="font-family: 微软雅黑;">等的快速酶切。</span></span></p> <p class="MsoNormal" style="margin-bottom: 0pt; line-height: 150%;"><strong><span style="font-family: Arial; line-height: 150%; color: #43227e; font-size: 12pt;"><span style="font-family: 微软雅黑;">产品</span></span></strong><strong><span style="font-family: Arial; line-height: 150%; color: #43227e; font-size: 12pt;"><span style="font-family: 微软雅黑;">特点</span></span></strong></p> <p class="MsoNormal" style="margin-bottom: 0.0000pt; margin-left: 42.0000pt; mso-para-margin-left: 0.0000gd; text-indent: -21.0000pt; mso-char-indent-count: 0.0000; text-autospace: ideograph-numeric; mso-pagination: widow-orphan; text-align: justify; text-justify: inter-ideograph; line-height: 150%; mso-list: l0 level1 lfo1;" align="justify"><span style="font-family: Wingdings; mso-fareast-font-family: 微软雅黑; mso-bidi-font-family: Arial; color: #191919; font-size: 12.0000pt;">Ø </span><span style="mso-spacerun: 'yes'; font-family: Arial; mso-fareast-font-family: 微软雅黑; line-height: 150%; color: #191919; font-size: 12.0000pt;">5~15 <span style="font-family: 微软雅黑;">分钟内即可完成酶切；</span></span></p> <p class="MsoNormal" style="margin-bottom: 0.0000pt; margin-left: 42.0000pt; mso-para-margin-left: 0.0000gd; text-indent: -21.0000pt; mso-char-indent-count: 0.0000; text-autospace: ideograph-numeric; mso-pagination: widow-orphan; text-align: justify; text-justify: inter-ideograph; line-height: 150%; mso-list: l0 level1 lfo1;" align="justify"><span style="font-family: Wingdings; mso-fareast-font-family: 微软雅黑; mso-bidi-font-family: Arial; color: #191919; font-size: 12.0000pt;">Ø </span><span style="mso-spacerun: 'yes'; font-family: Arial; mso-fareast-font-family: 微软雅黑; line-height: 150%; color: #191919; font-size: 12.0000pt;"><span style="font-family: 微软雅黑;">共用一种酶切</span> Buffer<span style="font-family: 微软雅黑;">，大大简化酶切反应体系；</span></span></p> <p class="MsoNormal" style="margin-bottom: 0.0000pt; margin-left: 42.0000pt; mso-para-margin-left: 0.0000gd; text-indent: -21.0000pt; mso-char-indent-count: 0.0000; text-autospace: ideograph-numeric; mso-pagination: widow-orphan; text-align: justify; text-justify: inter-ideograph; line-height: 150%; mso-list: l0 level1 lfo1;" align="justify"><span style="font-family: Wingdings; mso-fareast-font-family: 微软雅黑; mso-bidi-font-family: Arial; color: #191919; font-size: 12.0000pt;">Ø </span><span style="mso-spacerun: 'yes'; font-family: Arial; mso-fareast-font-family: 微软雅黑; line-height: 150%; color: #191919; font-size: 12.0000pt;"><span style="font-family: 微软雅黑;">良好的酶活冗余度，轻松应对底物过量或困难模板酶切</span></span><span style="mso-spacerun: 'yes'; font-family: Arial; mso-fareast-font-family: 微软雅黑; line-height: 150%; color: #191919; font-size: 12.0000pt;"><span style="font-family: 微软雅黑;">；</span></span></p> <p class="MsoNormal" style="margin-bottom: 0.0000pt; margin-left: 42.0000pt; mso-para-margin-left: 0.0000gd; text-indent: -21.0000pt; mso-char-indent-count: 0.0000; text-autospace: ideograph-numeric; mso-pagination: widow-orphan; text-align: justify; text-justify: inter-ideograph; line-height: 150%; mso-list: l0 level1 lfo1;" align="justify"><span style="font-family: Wingdings; mso-fareast-font-family: 微软雅黑; mso-bidi-font-family: Arial; color: #191919; font-size: 12.0000pt;">Ø </span><span style="mso-spacerun: 'yes'; font-family: Arial; mso-fareast-font-family: 微软雅黑; line-height: 150%; color: #191919; font-size: 12.0000pt;"><span style="font-family: 微软雅黑;">圣尔生物</span></span><span style="mso-spacerun: 'yes'; font-family: Arial; mso-fareast-font-family: 微软雅黑; line-height: 150%; color: #191919; font-size: 12.0000pt;"><span style="font-family: 微软雅黑;">去磷酸化、连接试剂在酶切</span> Buffer <span style="font-family: 微软雅黑;">中具有 </span>100% <span style="font-family: 微软雅黑;">活性，支持一管化反应，提升</span>“<span style="font-family: 微软雅黑;">酶切 </span>- <span style="font-family: 微软雅黑;">修饰 </span>- <span style="font-family: 微软雅黑;">连接</span>”<span style="font-family: 微软雅黑;">的体验。</span></span></p> <p class="MsoNormal" style="margin-bottom: 0pt; line-height: 150%;"><strong><span style="font-family: Arial; line-height: 150%; color: #43227e; font-size: 12pt;"><span style="font-family: 微软雅黑;">反应条件</span></span></strong></p> <p class="MsoNormal" style="margin-bottom: 0.0000pt; margin-left: 42.0000pt; mso-para-margin-left: 0.0000gd; text-indent: -21.0000pt; mso-char-indent-count: 0.0000; text-autospace: ideograph-numeric; mso-pagination: widow-orphan; text-align: justify; text-justify: inter-ideograph; line-height: 150%; mso-list: l0 level1 lfo1;" align="justify"><span style="font-family: Wingdings; mso-fareast-font-family: 微软雅黑; mso-bidi-font-family: Arial; color: #191919; font-size: 12.0000pt;">Ø </span><span style="mso-spacerun: 'yes'; font-family: Arial; mso-fareast-font-family: 微软雅黑; line-height: 150%; color: #191919; font-size: 12.0000pt;">1*<span style="font-family: 微软雅黑;">酶切缓冲液；</span></span></p> <p class="MsoNormal" style="margin-bottom: 0.0000pt; margin-left: 42.0000pt; mso-para-margin-left: 0.0000gd; text-indent: -21.0000pt; mso-char-indent-count: 0.0000; text-autospace: ideograph-numeric; mso-pagination: widow-orphan; text-align: justify; text-justify: inter-ideograph; line-height: 150%; mso-list: l0 level1 lfo1;" align="justify"><span style="font-family: Wingdings; mso-fareast-font-family: 微软雅黑; mso-bidi-font-family: Arial; color: #191919; font-size: 12.0000pt;">Ø </span><span style="mso-spacerun: 'yes'; font-family: Arial; mso-fareast-font-family: 微软雅黑; line-height: 150%; color: #191919; font-size: 12.0000pt;">37℃<span style="font-family: 微软雅黑;">温育。</span></span></p> <p class="MsoNormal" style="margin-bottom: 0pt; line-height: 150%;"><strong><span style="font-family: Arial; line-height: 150%; color: #43227e; font-size: 12pt;"><span style="font-family: 微软雅黑;">失活条件</span></span></strong></p> <p class="MsoNormal" style="margin-bottom: 0.0000pt; mso-para-margin-left: 2.0000gd; text-autospace: ideograph-numeric; mso-pagination: widow-orphan; line-height: 150%;"><span style="mso-spacerun: 'yes'; font-family: 微软雅黑; line-height: 150%; color: #191919; font-size: 12.0000pt;"> 80℃温育 20 min。</span></p> <p class="MsoNormal" style="margin-bottom: 0.0000pt; text-indent: 13.0000pt; text-align: justify; text-justify: inter-ideograph; line-height: 13.9500pt; mso-line-height-rule: exactly;" align="justify"> </p> <table style="border-collapse: collapse; width: 565px; height: 177px;" border="0" cellspacing="0" cellpadding="0"> <tbody> <tr style="mso-height-source: userset; height: 28.5pt;"> <td class="xl70" style="height: 28.5pt; width: 388pt;" colspan="3" width="516" height="38"><span><strong>圣尔快速内切酶在其它品牌的快酶buffer里的酶活情况</strong></span></td> </tr> <tr style="mso-height-source: userset; height: 26.5pt;"> <td class="xl67" style="height: 26.5pt; border-top: none;" height="35"><span style="color: #ff0000;"><span class="font7">ThermoFisher</span><span class="font8">的FD buffer</span></span></td> <td class="xl69" style="border-top: none; border-left: none;"><span style="color: #ff00ff;"><span class="font7">NEB</span><span class="font5">的cutsmart bufer</span></span></td> <td class="xl68" style="border-top: none; border-left: none;"><span style="color: #0000ff;"><span class="font7">Takara</span><span class="font8">的QuickCut buffer</span></span></td> </tr> <tr style="mso-height-source: userset; height: 26.5pt;"> <td class="xl65" style="height: 26.5pt; border-top: none; text-align: left;" height="35">100%活性</td> <td class="xl65" style="border-top: none; border-left: none;">100%活性</td> <td class="xl65" style="border-top: none; border-left: none;">100%活性</td> </tr> <tr style="mso-height-source: userset; height: 42.5pt;"> <td class="xl66" style="height: 42.5pt; width: 388pt;" colspan="3" width="516" height="57"><strong>100%活性表示圣尔生物的该酶可以在对应品牌的buffer里有效酶切，非100%活性则不可以。</strong></td> </tr> </tbody> </table> |
|---|---|
| 储存条件: | -20℃储存，4度蓝冰运输 |
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