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Product Name
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Recombinant Human Interferon-alpha1b
(rHuIFN-α1b)
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Cat. No.
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GH029
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Source
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Expressed in E. coli.
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Molecular weight
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Approximately 19.5kDa, a single non-glycosylated polypeptide chain containing 167 amino acids
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Accession
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P01562 Cys24-Glu189 with an N-terminal Met(A115V)
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Protein amino acid sequence
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MCDLPETHSLDNRRTLMLLAQMSRISPSSCLMDRHDFGFPQEEFDGNQFQKAPAISVLHELIQQIFNLFTTKDSSAAWDEDLLDKFCTELYQQLNDLEACVMQEERVGETPLMNVDSILAVKKYFRRITLYLTEKKYSPCAWEVVRAEIMRSLSLSTNLQERLRRKE
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Purity
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＞98% by SDS-PAGE or HPLC
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Formulation
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Lyophilized from a 0.2μm filtered solution in 20mMPB,300mMNaCl, 5%trehalose 0.01%tween-80 1mMDTT.
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Biological activity
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Fully biologically active when compared to standard. The specific activity determined by an anti-viral assay is no less than 1.0 ×108 IU/mg.
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Endotoxin level
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＜0.1EU/μg rHuIFN-α1b protein
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Reconstitution
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Before use this product, please read the direction below carefully.
1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.
2、 Reconstitute in a sterile aqueous buffer to an appropriate concentration.
Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.
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Storage/ Stability
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White lyophilized powder. For long term storage, the product should be stored ≤-20℃.
36 months, -20 to -70℃ as supplied;
1 month, 2 to 8℃ under sterile conditions after reconstitution;
3 months, -20 to -70℃ under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.
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THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.
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