| 产品详情 |
Epidermal growth factor (EGF) is a protein that stimulates cell growth and differentiation by binding to its receptor, EGFR. It was originally discovered in the submaxillary glands of mice and in human urine, and then has been found in many human tissues. EGF stimulates the proliferation of various epidermal and epithelial cells, inhibits gastric secretion, and involves in wound healing. Mature human EGF shares 70% a.a. sequence identity with mature mouse and rat EGF. Recombinant human EGF,1-51a.a is a single non-glycosylated polypeptide chain containing 51 amino acids，there are 3 intramolecular disulfide bonds in the EGF polypeptide chain and the molecular weight of EGF is 6.0kDa.
u Product Information
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Product Name
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Recombinant Human Epidermal Growth Factor, 1-51a.a
（rHuEGF,1-51a.a）
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Synonyms
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Epidermal Growth Factor, Urogastrone, URG
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Source
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Yeast
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Molecular weight
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Approximately 6.0kDa, a single glycosylated polypeptide chain containing 51 amino acids
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Accession
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P01133 Asn971-Arg1023
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AA sequence
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NSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWE
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Purity
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＞95% by SDS-PAGE or HPLC
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Biological activity
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Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1ng/ml, corresponding to a specific activity of >1.0 × 106 IU/mg
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Formulation
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Lyophilized from a 0.2 µm filtered solution in PBS, pH 7.4
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Endotoxin level
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＜0.01EU/μg rHuEGF,1-51a.a protein
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Reconstitution
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Before use this product, please read the direction below carefully.
1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.
2、 Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml.
Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
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Storage/ Stability
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For long term storage, the product should be stored ≤-20℃.
36 months, -20 to -70℃ as supplied.
1 month, 2 to 8℃ under sterile conditions after reconstitution;
3 months, -20 to -70℃ under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.
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THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.
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