| 产品详情 |
Parathyroid hormone (PTH) is a single polypeptide of 84 amino acids. PTH is a critical hormone in the regulation of Ca2+ homeostasis. PTH is secreted by the parathyroid glands, which promote release of calcium from bone to extracellular fluid by activating osteoblasts and inhibiting osteoclasts, indirectly promote increased intestinal absorption of calcium, and promote renal tubular reabsorption of calcium and increased renal excretion of phosphates. It is a major regulator of bone metabolism. Secretion of parathyroid hormone increases when the level of calcium in the extracellular fluid is low. PTH (7-34), which is a PTH/PTHrP receptor antagonist, can stimulate hair growth and epidermal proliferation in mice. Recombinant PTH7-34 is a 3.4kDa protein containing 28 amino acid residues.
u Product Information
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Product Name
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Recombinant Human Parathyroid Hormone 7-34
(rHuPTH7-34)
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Cat. No.
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GH048
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Source
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Expressed in E. coli.
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Molecular weight
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Approximately 3.4kDa, a single non-glycosylated polypeptide chain containing 28 amino acids.
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Accession
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P01270 Leu38-Phe65
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AA Sequence
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LMHNLGKHLNSMERVEWLRKKLQDVHNF
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Purity
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＞98% by SDS-PAGE or HPLC
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Formulation
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Lyophilized from a 0.2μm filtered solution in PBS pH7.4.
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Biological activity
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Fully biologically active when compared to standard. The specific activity is determined by UMR106 cell/cAMP method, corresponding to a specific activity of >1.0 × 104IU/mg.
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Endotoxin level
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＜0.1 EU/μg rHuPTH7-34 protein as determined by LAL method.
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Reconstitution
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Before use this product, please read the direction below carefully.
1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.
2、 Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml.
Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
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Storage/ Stability
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For long term storage, the product should be stored ≤-20℃.
36 months, -20 to -70℃ as supplied.
1 month, 2 to 8℃ under sterile conditions after reconstitution;
3 months, -20 to -70℃ under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.
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THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.
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