| 产品详情 |
3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGRC) Catalyzes the conversion of (3S)-hydroxy-3-methylglutaryl-CoA (HMG-CoA) to mevalonic acid, the rate-limiting step in the synthesis of cholesterol and other isoprenoids, thus plays a critical role in cellular cholesterol homeostasis. HMGCR is the main target of statins, a class of cholesterol-lowering drugs. Inhibition of HMGCR in the liver stimulates the LDL-receptors, which results in an increased clearance of LDL from the bloodstream and a decrease in blood cholesterol levels, The first result can be seen after one week of statin use and the effect is maximal after four to six weeks. Recombinant human HMGR is a 60.4kDa protein containing 560 amino acid residues.
u Product Information
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Product Name
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Recombinant human 3-Hydroxy-3-methylglutaryl-CoA Reductase, catalytic domain
(rHuHMGR)
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Cat. No.
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GH097
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Synonyms
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HMG-CoA reductase
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Source
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E. coli
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Molecular weight
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Approximately 60.4 kDa, a single chain containing 560 amino acids
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Accession
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P04035 Glu340-Ala888 with a c-terminal flag tag
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Amino Acid Sequence
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EQTETESTLSLKNPITSPVVTQKKVPDNCCRREPMLVRNNQKCDSVEEETGINRERKVEVIKPLVAETDTPNRATFVVGNSSLLDTSSVLVTQEPEIELPREPRPNEECLQILGNAEKGAKFLSDAEIIQLVNAKHIPAYKLETLMETHERGVSIRRQLLSKKLSEPSSLQYLPYRDYNYSLVMGACCENVIGYMPIPVGVAGPLCLDEKEFQVPMATTEGCLVASTNRGCRAIGLGGGASSRVLADGMTRGPVVRLPRACDSAEVKAWLETSEGFAVIKEAFDSTSRFARLQKLHTSIAGRNLYIRFQSRSGDAMGMNMISKGTEKALSKLHEYFPEMQILAVSGNYCTDKKPAAINWIEGRGKSVVCEAVIPAKVVREVLKTTTEAMIEVNINKNLVGSAMAGSIGGYNAHAANIVTAIYIACGQDAAQNVGSSNCITLMEASGPTNEDLYISCTMPSIEIGTVGGGTNLLPQQACLQMLGVQGACKDNPGENARQLARIVCGTVMAGELSLMAALAAGHLVKSHMIHNRSKINLQDLQGACTKKTAGGGSDYKDDDK
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Purity
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＞95% by SDS-PAGE under reducing conditions.
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Formulation
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10mM PB, pH 7.40，50% glycerol，2.5mM DTT
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Appearance
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Sterile Filtered colorless liquid
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Endotoxin level
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＜0.5EU/ug of rHuHMGR as determined by LAL method
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Storage/ Stability
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Protein solution is stable at -20°C for 6 months to 12 months.
Keep at -80°C for long term storage.
Avoid repeated freeze/thaw cycles after reconstitution.
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THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.
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