| 产品详情 |
Chemokine (C-C motif) ligand 1(CCL1) belongs to a family inflammatory cytokine (also known as chemokines). It is a small glycoprotein secreted by activated T cells with a molecular weight of approximately 8.5kDa. CCL1 signals through the CCR8 receptor. Human CCL1 has been assumed to be a homologue of the mouse TCA3. While the two proteins share only approximately 42% amino acid sequence identity, both chemokines contain an extra pair of cysteine residues not found in most other chemokines. Recombinant Human I-309 is an 8.6kDa protein consisting of 74 amino acid residues that include six cysteine residues, of which four are the highly conserved cysteine residues present in CC chemokines. Unlike some CC chemokines, whose biologically active form in solution is a non-disulfide-linked dimer, the biologically active form of I-309 is monomeric.
u Product Information
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Product Name
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Recombinant human I-309/CCL1
(rHuI-309/CCL1)
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Synonyms
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Small-inducible cytokine A1, T lymphocyte-secreted protein I-309
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Source
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Expressed in E. coli.
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Molecular weight
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Approximately 8.6kDa, a single non-glycosylated polypeptide chain containing 74 amino acids.
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Accession
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P22362 Ser23-Lys96
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AA Sequence
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SKSMQVPFSRCCFSFAEQEIPLRAILCYRNTSSICSNEGLIFKLKRGKEACALDTVGWVQRHRKMLRHCPSKRK
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Purity
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＞98% by SDS-PAGE or HPLC.
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Biological activity
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Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 10-100 ng/ml.
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Formulation
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Lyophilized from a 0.2µm filtered concentrated solution in 20mMPB,150 mM NaCl, pH 7.4.
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Endotoxin level
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＜0.1EU/μg rHuI-309/CCL1 protein as determined by LAL method.
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Reconstitution
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Before use this product, please read the direction below carefully.
1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.
2、 Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/ml.
Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
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Storage/ Stability
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For long term storage, the product should be stored ≤-20℃.
36 months, -20 to -70℃ as supplied.
1 month, 2 to 8℃ under sterile conditions after reconstitution;
3 months, -20 to -70℃ under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.
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THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.
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