| 产品详情 |
CCL20, also named LARC (Liver and Activation-regulated Chemokine), is encoded by CCL20 located on chromosome 2 in humans. The expression can be induced by LPS and some inflammatory cytokines, and it is at a high level in lymph nodes, PBL, fatal liver, fatal lung, appendix and so on. CCL20 has functions of chemotactic towards lymphocytes and dendritic cells. Additionally, it promotes the adhesion of memory CD4+ T cells and inhibits colony formation of bone marrow myeloid immature progenitors. CCL20 elicits it effects by binding with CCR6 receptor. Recombinant Human MIP-3α is an 8.0kDa protein containing 70 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.
Product Information
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Product Name
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Recombinant Human Macrophage Inflammatory Protein-3 alpha/CCL20
(rHuMIP-3α/CCL20)
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Synonyms
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LARC, Beta-chemokine Exodus-1, Liver and Activation-regulated Chemokine
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Source
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Expressed in E. coli.
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Molecular weight
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Approximately 8.0kDa, a single non-glycosylated polypeptide chain containing 70 amino acids.
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Accession
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P78556 Ala27-Met96
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AA Sequence
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ASNFDCCLGYTDRILHPKFIVGFTRQLANEGCDINAIIFHTKKKLSVCANPKQTWVKYIVRLLSKKVKNM
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Purity
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＞97% by SDS-PAGE or HPLC
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Formulation
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Lyophilized from Lyophilized from a 0.2μm filtered solution in PBS，pH7.4.
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Biological activity
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Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 10-50ng/ml.
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Endotoxin level
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Less than 0.1EU/mg of rHuMIP-3α/CCL20 as determined by LAL method
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Reconstitution
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Before use this product, please read the direction below carefully.
1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.
2、 Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
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Storage/ Stability
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For long term storage, the product should be stored ≤-20℃. Please avoid repeated freeze-thaw cycles after reconstitution.
12 months from date of receipt, -20 to -70℃ as supplied.
1 month, 2 to 8℃ under sterile conditions after reconstitution.
3 months, -20 to -70℃ under sterile conditions after reconstitution.
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THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.
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