| 产品详情 |
CCL28 belongs to CC family chemokine, also named mucosae-associated epithelial chemokine (MEC). The CCL28 cDNA is located on Chr.5 in humans, and the mRNA of CCL28 is highly expressed by epithelial cells of diverse tissues including normal and pathological colon, salivary gland, mammary gland, trachea and rectum. The amino acids sequence of human and mouse CCL28 shares 83 % identity, when the protein is processed to be the mature form. CCL28 is signaling through CCR3 and CCR10, and chemotactic for resting CD4, CD8 T-cells and eosinophils. MEC/ CCL28 contains six cysteines, including the four highly conserved cysteine residues present in CC chemokines. Recombinant Human MEC/ CCL28 is a 12.3kDa protein containing 108 amino acid residues.
Product Information
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Product Name
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Recombinant Human mucosae-associated epithelial chemokine /CCL28
(rHuMEC/CCL28)
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Synonyms
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CCK1
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Source
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Expressed in E. coli.
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Molecular weight
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Approximately 12.3kDa, a single non-glycosylated polypeptide chain containing 108 amino acids.
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Accession
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Q9NRJ3 Ser20-Tyr127
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AA Sequence
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SEAILPIASSCCTEVSHHISRRLLERVNMCRIQRADGDCDLAAVILHVKRRRICVSPHNHTVKQWMKVQAAKKNGKGNVCHRKKHHGKRNSNRAHQGKHETYGHKTPY
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Purity
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＞96% by SDS-PAGE or HPLC
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Formulation
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Lyophilized from a 0.2mm filtered solution in PBS, pH 7.0.
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Biological activity
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Fully biologically active when compared to standard. Determined by its ability to chemoattract human lymphocytes using a concentration range of 1.0-10.0ng/ml
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Endotoxin level
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Less than 0.1EU/mg of rHuMEC/CCL28 as determined by LAL method
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Reconstitution
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Before use this product, please read the direction below carefully.
1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.
2、 Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml.
Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃.
Further dilutions should be made in appropriate buffered solutions.
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Storage/ Stability
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For long term storage, the product should be stored ≤-20℃.
36 months, -20 to -70℃ as supplied.
1 month, 2 to 8℃ under sterile conditions after reconstitution;
3 months, -20 to -70℃ under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.
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THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.
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