| 产品详情 |
Heparin-binding epidermal growth factor (HB-EGF)-like growth factor (EGF) is found in cerebral neurons. Its expression is increased after hypoxic or ischemic injury, which also stimulates neurogenesis. HB-EGF has been implicated as a participant in a variety of normal physiological processes such as blastocyst implantation, wound healing, and in pathological processes such as tumor growth, SMC hyperplasia and atherosclerosis. HB-EGF is an 87 amino acid mitogenic and chemotactic glycoprotein containing an EGF-like domain with six conserved cysteine residues. Mouse HB-EGF shares about 73% a.a. sequence identity with human HB-EGF. Recombinant murine HB-EGF is a 9.8kDa protein containing 86 amino acid residues.
u Product Information
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Product Name
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Recombinant Murine Heparin-binding EGF-like Growth Factor
(rMuHB-EGF)
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Synonyms
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HBEGF, DT-R
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Source
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Expressed in E. coli.
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Molecular weight
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Approximately 9.8kDa, a single non-glycosylated polypeptide chain containing 86 amino acids.
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Accession
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Q06186 Asp63-Leu148
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AA Sequence
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DLEGTDLNLFKVAFSSKPQGLATPSKERNGKKKKKGKGLGKKRDPCLRKYKDYCIHGECRYLQEFRTPSCKCLPGYHGHRCHGLTL
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Purity
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＞97% by SDS-PAGE or HPLC
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Formulation
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Lyophilized from a 0.2µm filtered concentrated solution in 10 mM PB, 500 mM NaCl, pH 7.4.
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Biological activity
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Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0 ×106U/mg.
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Endotoxin level
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＜0.01EU/μg rMuHB-EGF protein as determined by LAL method.
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Reconstitution
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Before use this product, please read the direction below carefully.
1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.
2、 Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/ml.
Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
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Storage/ Stability
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White lyophilized powder. For long term storage, the product should be stored ≤-20℃. Please avoid repeated freeze-thaw cycles after reconstitution.
12 months from date of receipt, -20 to -70℃ as supplied.
1 month, 2 to 8℃ under sterile conditions after reconstitution.
3 months, -20 to -70℃ under sterile conditions after reconstitution.
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THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.
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