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Product Name
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Recombinant Rat Oncostatin-M
(rMuOSM)
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Synonyms
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Oncostatin M; Oncostatin-M; OSM
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Source
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Expressed in E. coli.
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Molecular weight
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Approximately 24.4kDa, a single non-glycosylated polypeptide chain containing 215 amino acids.
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Accession
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Q65Z15 Lys26-Arg239
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AA Sequence
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KRGCSSSSPKLLSQLKSQANITGNTASLLEPYILHQNLNTLTLRAACTEHPVAFPSEDMLRQLSKPDFLSTVHATLGRVWHQLGAFRQQFPKIQDFPELERARQNIQGIRNNVYCMARLLHPPLEIPEPTQADSGTSRPTTTAPGIFQIKIDSCRFLWGYHRFMGSVGRVFEEWGDGSRRSRRHSPLWAWLKGDHRIRPSRSSQSAMLRSLVPR
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Purity
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＞96% by SDS-PAGE or HPLC.
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Biological activity
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Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine NIH-3T3 cells is less than 2.0ng/ml, corresponding to a specific activity of > 5.0×105 IU/mg.
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Formulation
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Supplied as a sterile solution in 2 × PBS, 0.1% Tween-20.
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Endotoxin level
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＜0.1EU/μg rMuOSM protein as determined by LAL method.
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Reconstitution
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Before use this product, please read the direction below carefully.
1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.
2、 Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/ml.
Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
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Storage/ Stability
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For long term storage, the product should be stored ≤-20℃.
36 months, -20 to -70℃ as supplied.
1 month, 2 to 8℃ under sterile conditions after reconstitution;
3 months, -20 to -70℃ under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.
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THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.
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