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Product Name
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Recombinant Rat Gamma-Interferon Inducible Protein 10/CXCL10
(rRtIP-10/CXCL10)
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Synonyms
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Gamma-IP10, Small-inducible Cytokine B10, Protein Mob-1
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Source
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Expressed in E. coli.
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Molecular weight
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Approximately 8.7kDa, a single non-glycosylated polypeptide chain containing 77 amino acids.
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Accession
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P48973 Ile22- Pro98
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AA Sequence
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IPLARTVRCTCIDFHEQPLRPRAIGKLEIIPASLSCPHVEIIATMKKNNEKRCLNPESEAIKSLLKAVSQRRSKRAP
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Purity
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＞96% by SDS-PAGE or HPLC.
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Biological activity
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Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR3 transfected HEK293 cells is in a concentration range of 10-50ng/ml.
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Formulation
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Lyophilized from a 0.2µm filtered concentrated solution in 20mMPB, 150mM NaCl, pH 7.4
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Endotoxin level
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＜0.01EU/μg rRtIP-10/CXCL10 protein as determined by LAL method.
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Reconstitution
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Before use this product, please read the direction below carefully.
1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.
2、 Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml.
Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
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Storage/ Stability
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White lyophilized powder. For long term storage, the product should be stored ≤-20℃
36 months, -20 to -70℃ as supplied.
1 month, 2 to 8℃ under sterile conditions after reconstitution;
3 months, -20 to -70℃ under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.
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THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.
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